By W. W. Christie
This can be the 3rd quantity of an occasional sequence of assessment volumes facing features of lipid technique. As with the 1st volumes, issues were chosen which have been constructing speedily lately and feature a few value to lipid research. The authors are all major overseas experts.
Topics lined contain: research of positional isomers of glycerolipids by way of non-enzymatic tools, separation of phospholipid sessions by means of high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy and lipid part behaviour and lipid diffusion, between others
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Extra info for Advances in Lipid Methodology. Volume 3
There is also a need to reexamine the conventional Grignard degradation for bias with a greater selection of standard triacylglycerols, and for increasing the yield of the diacylglycerols or monoacylglycerols, as required, and minimizing isomerization, especially of the monoacylglycerols. Perhaps, the ethylmagnesium bromide needs to be replaced by the more reactive allylmagnesium bromide as the Grignard reagent. Finally, the prefractionation of the triacylglycerols advocated for the stereospecific analyses by non-enzymatic methods might lead also to more meaningful positional analysis of the intact triacylglycerols by purely physical methods.
30, 432-437 (1995). Chapter2 NUCLEAR MAGNETIC RESONANCE PROFILING OF PHOSPHOLIPIDS 31P Thomas Glonek1 and Thomas E. Merchant2 1MR Laboratory, Midwestern University, Chicago, Illinois 60615; 2Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA. A. Introduction B. Total Lipid Extraction C. A 31 P NMR Phospholipid Reagent 1. A hydrated chloroform-methanol reagent for 31 P NMR analysis 2. Effect of phospholipid concentration on phosphorus chemical shifts 3.
Biochem. Biophys. Res. , 168, 506511 (1990). , Ohrui,H. , Biochim. Biophys. Acta, 1168, 253260 (1993). Wedmid,Y. , lipids, 11, 189-193 (1976). ,J. Am. Oil Chem. , 67, 487-494 (1990). ,J. Am. Oil Chem. , 68, 391-400 (1991 ). Yang,L-Y. ,J. , 32, 1173-1186 (1991). , Kuksis,A. J. , 31, 137-147 (1990). , Kuksis,A. ,J. , 36, 1046-1057 (1995). J. , J. , 36, 125-136 (1995). , Lorbeer,E. , Org. , 29, 253-259 (1994). Zollner,F. ,J. , 30, 432-437 (1995). Chapter2 NUCLEAR MAGNETIC RESONANCE PROFILING OF PHOSPHOLIPIDS 31P Thomas Glonek1 and Thomas E.
Advances in Lipid Methodology. Volume 3 by W. W. Christie