By Holst, Otto
Skilled researchers describe in step by step aspect robust equipment for the research of bacterial exo- and endotoxins. those cutting-edge recommendations diversity from purification and detection tools to tools of conformational research, and contain using phage antibody libraries and strategies of structural and useful analyses of endotoxins. every one protocol is defined through a scientist who has on a regular basis used the strategy and optimized it to a excessive point of functionality. broad notes take care of the problems which may come up whilst utilizing the tactic and with the method's boundaries and attainable amendment for different reasons. state-of-the-art and geared to generating winning experimental effects, Bacterial pollution: equipment and Protocols presents investigators with a major selection of comfortably reproducible tools designed to assist contemporary bacterial toxin investigators-both beginner and expert-utilize a wide range of strong study instruments.
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Additional info for Bacterial Toxins: Methods and Protocols (Methods in Molecular Biology Vol 145)
7. Bioassays Extreme caution should be taken when performing these procedures. The IV method will give toxicity data in 30–70 min with concentrated toxin solutions. The IP method is completed in 3–4 d, but requires less technical expertise to perform accurately. 1. IV Mouse Assay 1. 2% (w/v) gelatin (gel-phosphate buffer). 2. 1 mL of the diluted sample into the lateral tail veins of three to five animals. Record the time the animals were injected and mark the animals (see Note 13). 3. Record the time to death and determine the average time to death (ATTD) for the mice (see Note 14).
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Bacterial Toxins: Methods and Protocols (Methods in Molecular Biology Vol 145) by Holst, Otto