Download PDF by Holst, Otto: Bacterial Toxins: Methods and Protocols (Methods in

By Holst, Otto

ISBN-10: 0896036049

ISBN-13: 9780896036048

ISBN-10: 1592590527

ISBN-13: 9781592590520

Skilled researchers describe in step by step aspect robust equipment for the research of bacterial exo- and endotoxins. those cutting-edge recommendations diversity from purification and detection tools to tools of conformational research, and contain using phage antibody libraries and strategies of structural and useful analyses of endotoxins. every one protocol is defined through a scientist who has on a regular basis used the strategy and optimized it to a excessive point of functionality. broad notes take care of the problems which may come up whilst utilizing the tactic and with the method's boundaries and attainable amendment for different reasons. state-of-the-art and geared to generating winning experimental effects, Bacterial pollution: equipment and Protocols presents investigators with a major selection of comfortably reproducible tools designed to assist contemporary bacterial toxin investigators-both beginner and expert-utilize a wide range of strong study instruments.

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7. Bioassays Extreme caution should be taken when performing these procedures. The IV method will give toxicity data in 30–70 min with concentrated toxin solutions. The IP method is completed in 3–4 d, but requires less technical expertise to perform accurately. 1. IV Mouse Assay 1. 2% (w/v) gelatin (gel-phosphate buffer). 2. 1 mL of the diluted sample into the lateral tail veins of three to five animals. Record the time the animals were injected and mark the animals (see Note 13). 3. Record the time to death and determine the average time to death (ATTD) for the mice (see Note 14).

78. 79. 80. 81. 82. 83. 84. 23 superantigen erythrogenic toxins, heat-killed streptococci and lipopolysaccharide. Infect. Immun. 62, 4915–4921. , Alouf, J. , Ozegowski, J. -M. (1996) Human pro- and anti-inflammatory cytokine patterns induced by Streptococcus pyogenes erythrogenic (pyrogenic) exotoxin A and C superantigens. Infect. Immun. 64, 1450–1453. , Alouf, J. , and Capron, M. (1997) Streptococcal pyrogenic exotoxin A (SPE A) superantigen induced production of hematopoietic cytokines, IL-12 and IL-13 by human peripheral blood mononuclear cells.

Biol. 5, 898–902. 97. , and Sax, M. (1992) Crystal structure of staphylococcal enterotoxin B, a superantigen. Nature 359, 801–806. 98. Jardetzky, T. , Brown, J. , Gorga, J. , Stern, L. , Urban, R. , Chi, Y. , Strominger, J. , and Wiley, D. C. (1994) Three-dimensional structure of a human class II histocompatibility molecule complexed with superantigen. Nature 368, 711–718. 99. Hoffmann, M. , Jablonski, L. , Crum, K. , Hackett, S. , Chi, Y. , Stauffacher, C. , Stevens, D. , and Bohach, G. A. (1994) Predictions of T-cell Bacterial Protein Toxins 25 receptor- and major histocompatibility complex-binding sites on staphylococcal enterotoxin C1.

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Bacterial Toxins: Methods and Protocols (Methods in Molecular Biology Vol 145) by Holst, Otto

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