Download e-book for kindle: Baculovirus and Insect Cell Expression Protocols by David W. Murhammer (auth.), David W. Murhammer (eds.)

By David W. Murhammer (auth.), David W. Murhammer (eds.)

ISBN-10: 1588295370

ISBN-13: 9781588295378

Baculovirus Expression Protocols is an in depth advisor for utilizing the baculovirus expression vector method (BEVS) and/or insect cells to supply recombinant proteins. systems for the advance and creation of baculovirus pesticides at either laboratory and big scale are defined within the type of step by step structure that the equipment in Molecular Biology™ sequence helped standardize. This booklet is a one-stop resource for info on baculoviruses, and comprises sections on utilizing changed baculoviruses to specific genes in mammalian cells, utilizing Drosophila mobilephone traces, keeping apart new mobilephone strains, and constructing serum-free medium. particular purposes, together with utilizing baculovirus and bug mobilephone method to review apoptosis, also are mentioned. This up-to-date and increased variation of Baculovirus Expression Protocols presents an ideal lab guide for biochemists, molecular biologists, biochemical engineers, and others for utilizing the BEVS or insect mobile traces to supply recombinant proteins.

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Volkman, L. , Goldsmith, P. , Hess, R. , and Faulkner, P. (1984) Neutralization of budded Autographa californica NPV by a monoclonal antibody: identification of the target antigen. Virology 133, 354–362. Blissard, G. , and Rohrmann, G. F. (1989) Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170, 537– 555. Pearson, M. , and Rohrmann, G. F. (2000) Identification of the Lymantria dispar nucleopolyhedrovirus envelope fusion protein provides evidence for a phylogenetic division of the Baculoviridae.

16 16. 17 17. 18 18. 19 19. 20 20. 21 21. 43 neutralization, comparative infectivity, and in vitro growth studies. J. Virol. 19, 820–832. Volkman, L. E. (1983) Occluded and budded Autographa californica nuclear polyhedrosis virus: Immunological relatedness of structural proteins. J. Virol. 46, 221–229. Volkman, L. E. and Goldsmith, P. A. (1984) Budded Autographa californica NPV 64K protein: Further biochemical analysis and effects of postimmunoprecipitation sample preparation conditions. Virology 139, 295.

K. (1988) The location, sequence, 72. transcription, and regulation of a baculovirus DNA polymerase gene. Virology 167, 591–600. 73 Lu, A. and Carstens, E. B. (1993) Immediate-early baculovirus genes transactivate 73. the p143 gene promoter of Autographa californica nuclear polyhedrosis virus. Virology 195, 710–718. 74 Kogan, P. H. and Blissard, G. W. (1994) A baculovirus gp64 early promoter is 74. activated by host transcription factor binding to CACGTG and GATA elements. J. Virol. 68, 813–822.

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Baculovirus and Insect Cell Expression Protocols by David W. Murhammer (auth.), David W. Murhammer (eds.)

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