By Arvid B. Maunsbach, Björn A. Afzelius
This finished reference illustrates optimum instruction tools in organic electron microscopy in comparison with universal methodological difficulties. not just will the fundamental methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be awarded, however the authors additionally pastime to demonstrate extra really expert concepts reminiscent of unfavourable staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted photograph analysis.
- Authored by means of the main leaders within the organic electron microscopy field
- Illustrates either optimum and suboptimal or artifactual leads to a number of electron microscopy disciplines
- Introduces scholars on the way to learn and interpret electron micrographs
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Extra info for Biomedical electron microscopy : illustrated methods and interpretations
It is impossible to label one pattern as the true representation of the cellular organization and the others as false. Additional physiological, physical, or chemical data are necessary in order to evaluate the significance of the observed patterns. Such information may be obtained from cryosectioning, freeze-fracturing, or tracer experiments. 2. FIXATIVES 35 36 2. 1 M sodium cacodylate buffer, postfixed in 1% osmium tetroxide, embedded in Epon, and section stained with uranyl acetate and lead citrate.
In neither case is it possible to arrive at firm conclusions solely on the basis of the observed structural pattern. The cell within an opening of the endothelium, possibly a lymphocyte, may be in transit between the space of Disse (left) to the sinusoid (right), as the cell appears to have pseudopodia on its right part. However, this is only a guess, and further experimental studies using radioactively labeled cell or vital microscopy may answer this question. Vesicles in contact with the cell membrane may be interpreted as showing exocytosis or endocytosis, or they may be stationary caveolae.
Electron microscopy of human immunodeficiency virus. J. Gen. Virol. 69, 2455-2469. Johannessen, J. V. (1977). Use of paraffin material for electron microscopy. Pathol. Annu. 12, 189-224. JCrgensen, P. L. (1974). Purification and characterization of (Na+-K+) ATPase. III. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphate. Biochim. Biophys. Acta 356, 36-52. , and Afzelius, B. (1962). A case of severe hypermetabolism of nonthyroid origin with a defect in the maintenance of mitochondrial respiratory control: A correlated clinical, biochemical, and morphological study.
Biomedical electron microscopy : illustrated methods and interpretations by Arvid B. Maunsbach, Björn A. Afzelius