By John N. Abelson, Melvin I. Simon, Helmut Sies
The seriously acclaimed laboratory normal for 40 years, equipment in Enzymology is without doubt one of the such a lot hugely revered guides within the box of biochemistry. considering 1955, each one quantity has been eagerlyawaited, usually consulted, and praised through researchers and reviewers alike. greater than 250 volumes were released (all of them nonetheless in print) and lots more and plenty of the cloth is appropriate even today--truly a necessary ebook for researchers in all fields of lifestyles sciences. Key positive aspects* Distribution, biosynthesis, metabolism, and delivery of glutathione* sign transduction and gene legislation* Thioredoxin and glutaredoxin* Mimics of organic thiols and thiol inhibitors
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Extra info for Biothiols, Part B: Glutathione and Thioredoxin: Thiols in Signal Transduction and Gene Regulation
Schaich, and F. Schneider, Hoppe-Seyler's Z. Physiol. Chem. 352, 159 (1971). 49L. Floh6 and W. A. Giinzler, in "Glutathione" (L. Floh& H. C. BenOhr, H. Sies, H. D. Waller, and A. ), p. 132. Thieme, Stuttgart, 1974. 5oL. Floh6, W. A. Gtinzler, and H. H. Schock, FEBS Lett. 32, 132 (1973). 51O. Epp, R. Ladenstein, and A. Wendel, Eur. J. Biochem. 133, 51 (1983). 52R. Ladenstein, O. Epp, W. A. Giinzler, and L. Floh4, Life Chem. Rep. 4, 37 (1986). 53F. Ursini, M. Maiorino, and C. Gregolin, Bioehim. Biophys.
Selig and A. Meister, this series, Vol. 113, p. 379. 2 p. Richman and A. Meister, J. Biol. Chem. 250, 1422 (1975). -S. -S. Chang, M. E. Anderson, and A. Meister, J. Biol. Chem. 268, 19675 (1993). 252 Copyright© 1995by AcademicPress,Inc. All fightsof reproductionin any formreserved.  GLUTATHIONE BIOSYNTHESISAND ITS INHIBITION 27 and sequenced have indicated that both are essential. 3 The latter is readily distinguishable from E. coli ~/-glutamylcysteine synthetase by making use of the fact that the kidney enzyme is markedly inhibited by cystamine.
Alternatively, an intramolecular disulfide pathway can displace the reduction product, ascorbic acid, followed by exchange with 2 mol of GSH successively. [From W. W. Wells, Y. Yang, T. L. -R. Gan, in "Advances in Enzymology and Related Areas of Molecular Biology" (A. ), Volume 66, p. 149. Copyright © 1993 John Wiley & Sons, Inc. 5, and 20% (v/v) glycerol at - 7 0 ° is stable for several months. Analysis of the enzyme preparation by S D S P A G E show that it is free of material with a molecular weight of about 12,000.
Biothiols, Part B: Glutathione and Thioredoxin: Thiols in Signal Transduction and Gene Regulation by John N. Abelson, Melvin I. Simon, Helmut Sies