Download PDF by Reinhard Kuhn, Sabrina Hoffstetter-Kuhn: Capillary Electrophoresis: Principles and Practice

By Reinhard Kuhn, Sabrina Hoffstetter-Kuhn

ISBN-10: 3540564349

ISBN-13: 9783540564348

Capillary electrophoresis (CE) is a brand-new analytical approach with the potential of fixing many analytical separation difficulties very quickly and economically. this system provides new information regarding the investigated components which can't simply be got by means of different capability. CE has develop into a longtime technique only in the near past, yet may be carried out in nearly each analytical laboratory in undefined, provider devices and academia within the close to destiny. crucial fields of CE program are pharmaceutical and biochemical learn and quality controls. The authors have exhaustive functional event within the program of CE tools within the pharmaceutical and supply the reader with a finished remedy of this technique. the focus is on how one can resolve difficulties whilst using CE within the laboratory. Physico-chemical thought is just handled intensive whilst essential to comprehend the underlying separation mechanisms that allows you to remedy your difficulties on the analytical bench. An addendum comprises tables at the practise of buffers and advised extra analyzing.

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A unifonn flow velocity vector across the tube occurs. Only in the double layer Electroosmosis 23 region, very close to the capillary surface, the flow velocity approaches zero (see Pig. b). Zone broadening caused by the laminar flow profile in HPLC is therefore negligible in CZE . :::',:: ,':::.. '. .. : . : ',. '. :: . / fused sUica capillary \ Fig. 9. Flow profiles of pressure-driven systems (a) and electrically-driven systems (b). Arrows indicate flow velocity vectors The reason for the relative motion of the buffer solution can be found in the nature of the capillary surface which can be described by Stem's model of the double layer (Fig.

2. For further information about thermal effects, temperature control and calculation of internal temperatures see Refs. 21 and 32 - 40. The influence of column temperature on the selectivity of the separation is treated in Sect. 3. 4 Electrophoretic Dispersion Changes in concentrations during electrophoresis are restricted by Koh1rausch's "beharrliche Funktion", regulating function or omega function [1] which results from the fact that all electrophoretic processes are essentially charge-transport processes obeying Ohm's law.

The trailing boundary, however, broadens with time and has a decreasing velocity: if the sample constituent enters the rear phase of the surrounding buffer, its velocity will also be decreased, thus resulting in a diffuse rear boundary of the zone. The peak shows a pronounced tailing and the peak height is decreased. b) Ils > IlB: If the mobility of the sample constituent is higher than that of the coion, the electric fIeld strength in the sample zone will be lower than in the buffer zone. When a sample molecule diffuses into the frontal buffer zone, its velocity will be increased by the higher field strength of the buffer zone.

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Capillary Electrophoresis: Principles and Practice by Reinhard Kuhn, Sabrina Hoffstetter-Kuhn


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