By Diana P. Bratu (auth.), Douglas J. Taatjes, Brooke T. Mossman (eds.)
A various number of cutting-edge tools for the microscopic imaging of cells and molecules. The authors conceal a large spectrum of complimentary suggestions, together with such equipment as fluorescence microscopy, electron microscopy, atomic strength microscopy, and laser scanning cytometry. extra comfortably reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted picture research, laser-capture microdissection, microarray picture scanning, near-field scanning optical microscopy, and mirrored image distinction microscopy around out this eclectic selection of state-of-the-art imaging thoughts now on hand. The authors additionally speak about preparative tools for debris and cells via transmission electron microscopy.
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Additional resources for Cell Imaging Techniques: Methods and Protocols
2. , Lukins, P. , and Sheppard, C. J. R. (2001) Simultaneous multichannel nonlinear imaging: combined two-photon excited fluorescence and second harmonic generation microscopy. Micron 32, 685–689. 3. Hellwarth, R. and Christensen, P. (1974) Nonlinear microscopic examination of structure in polycrystalline ZnSe. Opt. Commun. 12, 318–322. 4. Gannaway, J. N. and Sheppard, C. J. R. (1978) Second harmonic imaging in the scanning optical microscope. Opt. Quant. Electron. 10, 435. 5. , Clark, H. , Mohler, W.
21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 45 Pipet tips and micropipets. 5-mL Eppendorf microcentrifuge tubes. Serological pipets and handheld dispenser (1, 5, 10, 25 mL). Fine-point jeweler’s forceps. Microperifusion system and chamber. Temperature-regulated water bath and thermometer. Peristaltic pump and tubing for perifusion system (various sizes; chemically resistant). Vibration-free isolation table for microscope. Pipette Aid motor or aquarium bubbler. Two 500-mL vacuum Ehrlenmeyer flasks and rubber stoppers and desiccant.
Hill, A. , Peters, C. , and Weinreich, G. (1961) Generation of optical harmonics. Phys. Rev. Lett. 7, 118–119. 2. , Lukins, P. , and Sheppard, C. J. R. (2001) Simultaneous multichannel nonlinear imaging: combined two-photon excited fluorescence and second harmonic generation microscopy. Micron 32, 685–689. 3. Hellwarth, R. and Christensen, P. (1974) Nonlinear microscopic examination of structure in polycrystalline ZnSe. Opt. Commun. 12, 318–322. 4. Gannaway, J. N. and Sheppard, C. J. R. (1978) Second harmonic imaging in the scanning optical microscope.
Cell Imaging Techniques: Methods and Protocols by Diana P. Bratu (auth.), Douglas J. Taatjes, Brooke T. Mossman (eds.)